Assessment of skin barrier function using mathematical modeling of TEWL-desorption curves: age related differences

  • Felipe Nunes Laboratório de Farmacotécnica, Universidade Federal do Paraná
  • Pedro Contreiras Pinto Universidade Lusófona
  • Luis Monteiro Rodrigues Universidade Lusófona
  • Catarina Rosado Universidade Lusófona

Resumo

The study aimed to investigate changes in the cutaneous barrier function caused by ageing using the POST (plastic
occlusion stress test) methodology followed by mathematical modeling of the TEWL data obtained after removal of the
occlusion.
32 healthy volunteers participated in the study. They were separated into two groups: one of volunteers under and the other
of subjects over 30 years old. An occlusive patch was applied to the ventral forearm of each volunteer for 2 hours. After
removal of the patch, the TEWL was measured for 30 minutes. A bi-compartmental mathematical model was fitted to the
data and t1/2evap – evaporation half-life- and DWM – dynamic water mass- were calculated.
Discrete differences were found between the kinetic parameters obtained for the two groups, which seems to indicate that
the water balance and skin barrier were not severely altered in the age ranges included in the study. Nevertheless, the
desorption curves of the under 30 volunteers presented a sharp decay, whereas in senile skin the decay occurred at a much
slower pace.
It can be concluded that the dynamic methodology was able to establish a mild differences in the water dynamics of aged
skin, which can potentially be applied to the efficacy and safety testing of skin products.
Publicado
Sep 22, 2010
Como citar
NUNES, Felipe et al. Assessment of skin barrier function using mathematical modeling of TEWL-desorption curves: age related differences. Revista Lusófona de Ciências e Tecnologias da Saúde, [S.l.], n. 2, sep. 2010. ISSN 1646-3854. Disponível em: <http://revistas.ulusofona.pt/index.php/revistasaude/article/view/2776>. Acesso em: 28 june 2017.
Edição
Secção
Ciências Biofarmacêuticas / Biopharmaceutical Sciences

Palavras-chave

barrier function; POST; TEWL; ageing.