QUANTIFYING MYOCARDIAL FIBROSIS ON MURINE MODEL USING IMAGEJ

Resumo

Quantification of histological images is a task often required in biomedical research. Many of the methods used are time-consuming, difficult to replicate and subjective. This work aimed to describe and validate a more objective, reproducible and easy to perform methodology to measure murine heart fibrosis, using ImageJ software. For this matter, a macro was created and validated for its intra and inter-observational reproducibility. No significant differences were found between the results obtained manually and with the macro (p> 0.05). Regarding the interobserver variability, the macro proved to be a less variable method by presenting higher correlation coefficients between observers when compared with the manual measurement.  With these results, we were able to define and describe a methodology that outputs objective and replicable results in a less laborious way.

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Publicado
Feb 18, 2019
Como citar
CATARINO, José et al. QUANTIFYING MYOCARDIAL FIBROSIS ON MURINE MODEL USING IMAGEJ. Revista Lusófona de Ciência e Medicina Veterinária, [S.l.], v. 9, p. 1-7, feb. 2019. Disponível em: <http://revistas.ulusofona.pt/index.php/rlcmv/article/view/6639>. Acesso em: 26 june 2019.
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