QUANTIFYING HEPATIC FIBROSIS ON MURINE MODELS: HOW TO OBTAIN REPRESENTATIVE RESULTS IN A LESS LABORIOUS WAY
In biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963).
Using ImageJ facilitated the definition of a methodology that originated representative results on a liver section and at the same time allowed objective measurement in a reproducible and less laborious way.