DNA DAMAGE IN CANINE TRANSMISSIBLE VENEREAL TUMOR CELLS

Anne Santos do Amaral; Isabelle Ferreira Ferreira; Marcia Moleta Colodel; Dayse Maria Fávero Salvadore; Noeme Sousa Rocha

doi:10.60543/rlcmv.v4i0.2388

Anne Santos do Amaral Department of Small Animal Clinic, UFSM, Santa Maria, RS, Brazil
Isabelle Ferreira Ferreira Department of Clinical Veterinary Medicine, FMVZ/UNESP, Botucatu, SP, Brazil
Marcia Moleta Colodel Department of Clinical Veterinary Medicine, FMVZ/UNESP, Botucatu, SP, Brazil
Dayse Maria Fávero Salvadore Department of Pathology, FMB/UNESP, Botucatu, SP, Brazil
Noeme Sousa Rocha Department of Clinical Veterinary Medicine, FMVZ/UNESP, Botucatu, SP, Brazil

DOI: https://doi.org/10.60543/rlcmv.v4i0.2388

Resumo

The Transmissible Venereal Tumor (TVT) has been classified according to the predominant cell type as follows: lymphocytoid, plasmocytoid, and mixed. Various degrees of aggressiveness with large array of biological behaviour have been described according to the TVT cell lineages, the present study was designed to investigate the level of DNA damage in the three TVT cell types aiming a better understanding of mechanisms related to the aggressiveness of this neoplasia. A total of 35 dogs were evaluated, with no restriction regarding sex, age or breed, and with clinical and cytological diagnoses for TVT. Cell samples from the 35 tumoral neoplasmas were obtained by fine needle aspiration and taken to cytology and DNA damage analysis by the comet assay. From the 35 TVT cases, 12 (34.3%) were plasmocytoid, 11 (31.4%) lymphocytoid, and 12 (34.3%) mixed. Statistically significant (p<0.05) higher level of DNA damage was detected in lymphocytoid TVT cells when compared to the other two types.

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