QUANTIFYING HEPATIC FIBROSIS ON MURINE MODELS: HOW TO OBTAIN REPRESENTATIVE RESULTS IN A LESS LABORIOUS WAY

Resumo

In biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963).


Using ImageJ facilitated the definition of a methodology that originated representative results on a liver section and at the same time allowed objective measurement in a reproducible and less laborious way.

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Publicado
Feb 18, 2019
Como citar
CATARINO, José et al. QUANTIFYING HEPATIC FIBROSIS ON MURINE MODELS: HOW TO OBTAIN REPRESENTATIVE RESULTS IN A LESS LABORIOUS WAY. Revista Lusófona de Ciência e Medicina Veterinária, [S.l.], v. 9, p. 17-22, feb. 2019. Disponível em: <https://revistas.ulusofona.pt/index.php/rlcmv/article/view/6658>. Acesso em: 18 aug. 2019.
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